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propidium iodide fluorescence intensity  (Miltenyi Biotec)
99
Miltenyi Biotec propidium iodide fluorescence intensity
A. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 TERT + cell lines on one hand and for the 3 ALT + cell lines on the other hand. For each cell line 1200 cells were seeded and proliferation was assessed at days 3, 4 and 5 (Fig. S10). Results presented here show the mean cell count on day 5 ± SD. Mann-Whitney test. ***: p < 0.001. B. Stacked bar chart presenting the result of three independent experiments in which cell fractions in G1, S and G2/M phases of the cell cycle were assessed using <t>propidium</t> iodide labelling for 2 TERT + hybrid cell lines and 3 ALT + cell lines. HBT3 TERT + cell line could not be analyzed because of the presence of two populations with different ploidies. Results correspond to the mean ± SD. Two-way ANOVA with Sidak’s correction for multiple comparisons test. **: p < 0.01 and ****: p < 0.0001. Individual data for each cell line are presented in Fig. S11. C. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 control cell lines (-DOX, in red) and for the 3 cell lines under DOX treatment (+DOX, in blue). Two shRNAs were used (sh2 & sh3). Results are mean ± SD. Unpaired t-test with Welch’s correction. No significant result. BLM inhibition induced by DOX treatment was assessed in parallel by real-time quantitative RT-PCR and western blot (Figs. S12A and S12B). Individual data for each cell line are presented in Fig. S12C.
Propidium Iodide Fluorescence Intensity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/propidium iodide fluorescence intensity/product/Miltenyi Biotec
Average 99 stars, based on 1 article reviews
propidium iodide fluorescence intensity - by Bioz Stars, 2026-02
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propidium iodide solution  (Miltenyi Biotec)
99
Miltenyi Biotec propidium iodide solution
A. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 TERT + cell lines on one hand and for the 3 ALT + cell lines on the other hand. For each cell line 1200 cells were seeded and proliferation was assessed at days 3, 4 and 5 (Fig. S10). Results presented here show the mean cell count on day 5 ± SD. Mann-Whitney test. ***: p < 0.001. B. Stacked bar chart presenting the result of three independent experiments in which cell fractions in G1, S and G2/M phases of the cell cycle were assessed using <t>propidium</t> iodide labelling for 2 TERT + hybrid cell lines and 3 ALT + cell lines. HBT3 TERT + cell line could not be analyzed because of the presence of two populations with different ploidies. Results correspond to the mean ± SD. Two-way ANOVA with Sidak’s correction for multiple comparisons test. **: p < 0.01 and ****: p < 0.0001. Individual data for each cell line are presented in Fig. S11. C. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 control cell lines (-DOX, in red) and for the 3 cell lines under DOX treatment (+DOX, in blue). Two shRNAs were used (sh2 & sh3). Results are mean ± SD. Unpaired t-test with Welch’s correction. No significant result. BLM inhibition induced by DOX treatment was assessed in parallel by real-time quantitative RT-PCR and western blot (Figs. S12A and S12B). Individual data for each cell line are presented in Fig. S12C.
Propidium Iodide Solution, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/propidium iodide solution/product/Miltenyi Biotec
Average 99 stars, based on 1 article reviews
propidium iodide solution - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
propidium iodide  (Miltenyi Biotec)
99
Miltenyi Biotec propidium iodide
A. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 TERT + cell lines on one hand and for the 3 ALT + cell lines on the other hand. For each cell line 1200 cells were seeded and proliferation was assessed at days 3, 4 and 5 (Fig. S10). Results presented here show the mean cell count on day 5 ± SD. Mann-Whitney test. ***: p < 0.001. B. Stacked bar chart presenting the result of three independent experiments in which cell fractions in G1, S and G2/M phases of the cell cycle were assessed using <t>propidium</t> iodide labelling for 2 TERT + hybrid cell lines and 3 ALT + cell lines. HBT3 TERT + cell line could not be analyzed because of the presence of two populations with different ploidies. Results correspond to the mean ± SD. Two-way ANOVA with Sidak’s correction for multiple comparisons test. **: p < 0.01 and ****: p < 0.0001. Individual data for each cell line are presented in Fig. S11. C. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 control cell lines (-DOX, in red) and for the 3 cell lines under DOX treatment (+DOX, in blue). Two shRNAs were used (sh2 & sh3). Results are mean ± SD. Unpaired t-test with Welch’s correction. No significant result. BLM inhibition induced by DOX treatment was assessed in parallel by real-time quantitative RT-PCR and western blot (Figs. S12A and S12B). Individual data for each cell line are presented in Fig. S12C.
Propidium Iodide, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/propidium iodide/product/Miltenyi Biotec
Average 99 stars, based on 1 article reviews
propidium iodide - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
propidium iodide pi  (Miltenyi Biotec)
99
Miltenyi Biotec propidium iodide pi
A. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 TERT + cell lines on one hand and for the 3 ALT + cell lines on the other hand. For each cell line 1200 cells were seeded and proliferation was assessed at days 3, 4 and 5 (Fig. S10). Results presented here show the mean cell count on day 5 ± SD. Mann-Whitney test. ***: p < 0.001. B. Stacked bar chart presenting the result of three independent experiments in which cell fractions in G1, S and G2/M phases of the cell cycle were assessed using <t>propidium</t> iodide labelling for 2 TERT + hybrid cell lines and 3 ALT + cell lines. HBT3 TERT + cell line could not be analyzed because of the presence of two populations with different ploidies. Results correspond to the mean ± SD. Two-way ANOVA with Sidak’s correction for multiple comparisons test. **: p < 0.01 and ****: p < 0.0001. Individual data for each cell line are presented in Fig. S11. C. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 control cell lines (-DOX, in red) and for the 3 cell lines under DOX treatment (+DOX, in blue). Two shRNAs were used (sh2 & sh3). Results are mean ± SD. Unpaired t-test with Welch’s correction. No significant result. BLM inhibition induced by DOX treatment was assessed in parallel by real-time quantitative RT-PCR and western blot (Figs. S12A and S12B). Individual data for each cell line are presented in Fig. S12C.
Propidium Iodide Pi, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/propidium iodide pi/product/Miltenyi Biotec
Average 99 stars, based on 1 article reviews
propidium iodide pi - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
stainedwith propidium iodide  (Miltenyi Biotec)
99
Miltenyi Biotec stainedwith propidium iodide
A. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 TERT + cell lines on one hand and for the 3 ALT + cell lines on the other hand. For each cell line 1200 cells were seeded and proliferation was assessed at days 3, 4 and 5 (Fig. S10). Results presented here show the mean cell count on day 5 ± SD. Mann-Whitney test. ***: p < 0.001. B. Stacked bar chart presenting the result of three independent experiments in which cell fractions in G1, S and G2/M phases of the cell cycle were assessed using <t>propidium</t> iodide labelling for 2 TERT + hybrid cell lines and 3 ALT + cell lines. HBT3 TERT + cell line could not be analyzed because of the presence of two populations with different ploidies. Results correspond to the mean ± SD. Two-way ANOVA with Sidak’s correction for multiple comparisons test. **: p < 0.01 and ****: p < 0.0001. Individual data for each cell line are presented in Fig. S11. C. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 control cell lines (-DOX, in red) and for the 3 cell lines under DOX treatment (+DOX, in blue). Two shRNAs were used (sh2 & sh3). Results are mean ± SD. Unpaired t-test with Welch’s correction. No significant result. BLM inhibition induced by DOX treatment was assessed in parallel by real-time quantitative RT-PCR and western blot (Figs. S12A and S12B). Individual data for each cell line are presented in Fig. S12C.
Stainedwith Propidium Iodide, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/stainedwith propidium iodide/product/Miltenyi Biotec
Average 99 stars, based on 1 article reviews
stainedwith propidium iodide - by Bioz Stars, 2026-02
99/100 stars
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A. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 TERT + cell lines on one hand and for the 3 ALT + cell lines on the other hand. For each cell line 1200 cells were seeded and proliferation was assessed at days 3, 4 and 5 (Fig. S10). Results presented here show the mean cell count on day 5 ± SD. Mann-Whitney test. ***: p < 0.001. B. Stacked bar chart presenting the result of three independent experiments in which cell fractions in G1, S and G2/M phases of the cell cycle were assessed using propidium iodide labelling for 2 TERT + hybrid cell lines and 3 ALT + cell lines. HBT3 TERT + cell line could not be analyzed because of the presence of two populations with different ploidies. Results correspond to the mean ± SD. Two-way ANOVA with Sidak’s correction for multiple comparisons test. **: p < 0.01 and ****: p < 0.0001. Individual data for each cell line are presented in Fig. S11. C. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 control cell lines (-DOX, in red) and for the 3 cell lines under DOX treatment (+DOX, in blue). Two shRNAs were used (sh2 & sh3). Results are mean ± SD. Unpaired t-test with Welch’s correction. No significant result. BLM inhibition induced by DOX treatment was assessed in parallel by real-time quantitative RT-PCR and western blot (Figs. S12A and S12B). Individual data for each cell line are presented in Fig. S12C.

Journal: bioRxiv

Article Title: Alternative Lengthening of Telomeres and CINSARC are interconnected toward non-translocation-related sarcomas progression

doi: 10.64898/2026.01.23.701253

Figure Lengend Snippet: A. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 TERT + cell lines on one hand and for the 3 ALT + cell lines on the other hand. For each cell line 1200 cells were seeded and proliferation was assessed at days 3, 4 and 5 (Fig. S10). Results presented here show the mean cell count on day 5 ± SD. Mann-Whitney test. ***: p < 0.001. B. Stacked bar chart presenting the result of three independent experiments in which cell fractions in G1, S and G2/M phases of the cell cycle were assessed using propidium iodide labelling for 2 TERT + hybrid cell lines and 3 ALT + cell lines. HBT3 TERT + cell line could not be analyzed because of the presence of two populations with different ploidies. Results correspond to the mean ± SD. Two-way ANOVA with Sidak’s correction for multiple comparisons test. **: p < 0.01 and ****: p < 0.0001. Individual data for each cell line are presented in Fig. S11. C. The bar chart corresponds to the mean of three independent proliferation experiments for the 3 control cell lines (-DOX, in red) and for the 3 cell lines under DOX treatment (+DOX, in blue). Two shRNAs were used (sh2 & sh3). Results are mean ± SD. Unpaired t-test with Welch’s correction. No significant result. BLM inhibition induced by DOX treatment was assessed in parallel by real-time quantitative RT-PCR and western blot (Figs. S12A and S12B). Individual data for each cell line are presented in Fig. S12C.

Article Snippet: DNA content was quantified with propidium iodide fluorescence intensity by flow cytometry (MACSQuant VYB, Miltenyi Biotec) and analyzed using the cycle tool of the FlowJo software (RRID:SCR_008520, v10.10.0, FlowJo LLC) and GraphPad Prism (RRID:SCR_002798, v6.01, GraphPad Software Inc.) software.

Techniques: Cell Characterization, MANN-WHITNEY, Control, Inhibition, Quantitative RT-PCR, Western Blot